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Yes. American Airlines and Delta Air Lines fly nonstop from New York (LGA) to Tampa (TPA), several times daily. The flight takes about 2h 35m and covers 1,012 miles (1,628 km).
American Airlines, Delta Air Lines operate nonstop flights from New York to Tampa.
The nonstop flight from New York to Tampa takes ~2h 35m (approximately 1628 km).
Nonstop flights from New York to Tampa operate several times daily.