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Yes. Aerolineas Argentinas and Felix Airways fly nonstop from El Calafate (FTE) to Ushuaia (USH). The flight takes about 1h 14m and covers 352 miles (567 km).
Aerolineas Argentinas, Felix Airways operate nonstop flights from El Calafate to Ushuaia.
The nonstop flight from El Calafate to Ushuaia takes ~1h 14m (approximately 567 km).